A Terbium Sensitized Luminescence Method for the Assay of Flubiprofen in Pharmaceutical Formulations

Authors

Salma M.Z. Al-Kindy, Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box: 36, PC 123, Al-Khod, Muscat, Sultanate of Oman.
Arwa Al-Ghazali, Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box: 36, PC 123, Al-Khod, Muscat, Sultanate of Oman.
Fakhr Eldin O. Suliman, Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box: 36, PC 123, Al-Khod, Muscat, Sultanate of Oman.
Haidar A.J. Al-Lawati, Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box: 36, PC 123, Al-Khod, Muscat, Sultanate of Oman.

Abstract

A sensitive time-resolved luminescence method for the determination of flubiprofen (FLP) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of terbium (Tb3+ ) by the formation of a ternary complex with FLP in the presence of 4,7 diphenyl 1,10 phenanthroline (DPP) as co-ligand, and Tween-20 as surfactant. The signal for Tb-FLP-DPP was monitored at λex = 285 nm and λem = 552 nm. Optimum conditions for the formation of the complex in an aqueous system were TRIS buffer, pH 8.0, DPP (2.5Å~10−7 M), Tween-20 (0.30%) and 4Å~10-5 mol L-1 of Tb3+ which allowed the determination of 20–1000 ng mL-1 of FLP with a limit of detection (LOD) of 10 ng mL-1 . The relative standard deviations of the method ranged between 0.6 and 1.4% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assays of FLP in pharmaceutical formulations and spiked tap water samples with average recoveries of 87% – 95%.

Recommended Citation

Al-Kindy, Salma M.Z.; Al-Ghazali, Arwa; Suliman, Fakhr Eldin O.; and Al-Lawati, Haidar A.J. (2014) A Terbium Sensitized Luminescence Method for the Assay of Flubiprofen in Pharmaceutical Formulations, Sultan Qaboos University Journal For Science: Vol. 19: Iss. 1, 54-63.
DOI: https://doi.org/10.24200/squjs.vol19iss1pp54-63
Available at: https://squjs.squ.edu.om/squjs/vol19/iss1/4