Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography

Authors

Sana S. Al-Sinani, Department of Biology, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khoud, 123 Muscat, Sultanate of Oman
Elsadig A. Eltayeb, Department of Biology, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khoud, 123 Muscat, Sultanate of Oman

Abstract

Improved and simplified HPLC conditions for the determination and quantification of both the steroidal glycoalkaloid (solamargine) and its aglycone (solasodine) are described. The best isocratic conditions were developed using a C18 column and methanol in combination with an ammonium dihydrogen phosphate buffer. The isocratic conditions were shown to be more reproducible, less time consuming and to give sharper peaks (better separation). The effects of adjusting solvent: buffer ratio, buffer pH and buffer molarity were evaluated.

Recommended Citation

Al-Sinani, Sana S. and Eltayeb, Elsadig A. (2013) Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography, Sultan Qaboos University Journal For Science: Vol. 18: Iss. 1, 1-10.
DOI: https://doi.org/10.24200/squjs.vol18iss0pp1-10
Available at: https://squjs.squ.edu.om/squjs/vol18/iss1/5